Publications
Selected Publications
 

Leyton, J.V., Olafsen, T., Lepin, E.J., Hahm, S., Bauer, K., Reiter, R.E., and Wu, A.M. A humanized radioiodinated minibody as an imaging agent for the detection of prostate stem cell antigen (PSCA)-expressing tumors by microPET. Clin. Canc. Res., in press.

Venisnik, K.M., Olafsen, T., Gambhir, S.S., and Wu, A.M. (2007) Fusion of Gaussia luciferase to an engineered anti-carcinoembryonic antigen (CEA) antibody for in vivo optical imaging. Mol. Imaging Biol., 9:267-277.

Barat, B. and Wu, A.M. (2007) Metabolic biotinyation of recombinant antibody by biotin ligase retained in endoplasmic reticulum. Biomolec. Eng. 24:283-291.

Olafsen, T., Gu, Z., Sherman, M.A., Leyton, J.V., Witkosky, M.E., Shively, J.E.., Raubitschek, A.A., Morrison, S.L., Wu, A.M. and Reiter, R.E. (2007) Targeting, imaging, and therapy using a humanized anti-prostate stem cell antigen (PSCA) antibody. J. Immunotherapy 30:396-405.

Cai, W., Olafsen, T., Zhang, X., Cao, Q., Gambhir, S.S., Williams, L.E., Wu, A.M. and Chen, X. (2007) PET imaging of colorectal cancer in xenograft-bearing mice by use of an 18F-labeled T84.66 anti-carcinoembryonic antigen diabody. J. Nucl. Med. 48:304-310.

Kenanova, V., Olafsen, T., Carter, N.H., Longmate, J., Chatziioannou, A.F., Williams, L.E., Shively, J.E., Colcher, D., Raubitschek, A.A. and Wu, A.M. (2007) Radioiodinated vs. radiometal labeled anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments: Optimal pharmacokinetics for therapy. Cancer Res. 67:718-726.

Olafsen, T., Kenanova, V.E., and Wu, A.M. (2006) Tunable pharmacokinetics: Modifying the in vivo half life of antibodies by directed mutagenesis of the Fc fragment. Nature Protocols 1:2048-2060.

Venisnik, K.M., Olafsen, T., Loening, A.M., Iyer, M., Gambhir, S.S., and Wu, A.M. (2006) Bifunctional antibody-Renilla luciferase fusion protein for in vivo optical detection of tumors. Prot. Eng. Des. Sel. 19:453-460.

Loening, A.M., Fenn, T.D., Wu, A.M. and Gambhir, S.S. (2006) Consensus guided mutagenesis of Renilla luciferase for increased stability and light output. Prot. Eng. Des. Sel. 19:391-400.

Kenanova, V.E. and Wu, A.M. (2006) Tailoring antibodies for radionuclide delivery. Exp. Opin. Drug Delivery 3:53-70.

Wu, A.M. and Senter, P.D. (2005) Arming antibodies: Prospects and challenges for immunoconjugates. Nature Biotechnology 23:1137-1146.

Olafsen, T., Kenanova, V., Sundaresan, G., Anderson, A.-L., Crow, D., Yazaki, P.J., Li, L., Press, M.F., Gambhir, .S.S., Williams, L.E., Wong, J.Y.C., Raubitschek, A.A., Shively, J.E., and Wu, A.M. (2005) Optimizing radiolabeled engineered anti-p185HER2 antibody fragments for in vivo imaging. Cancer Res. 65:5907-5916.

Michalet, X., Pinaud, F.F., Bentolila, L.A., Tsay, J.M., Doose, S., Li, J., Sundaresan, G., Wu, A.M., Gambhir, S.S., and Weiss, S. (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science,307:538-544.

Kenanova, V., Olafsen, T., Crow, D., Sundaresan, G., Subbarayan, Carter, N.H., Ikle, D.N., Yazaki, P.J., Chatziioannou, A.F., Gambhir, S.S., Williams, L.E., Shively, J.E., Colcher, D., Raubitschek, A. A., and Wu, A.M. (2005) Tailoring the pharmacokinetics and positron emission tomography imaging properties of anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments. Cancer Res. 65:622-631.

Wong, J.Y.C., Chu, D.Z., Williams, L.E., Yamauchi, D.M., Ikle, D.N., Kwok, C.S., Liu, A., Wilczynski, S., Colcher, D., Yazaki, J.E., Wu, A.M. andRaubitschek, A.A. (2004) Pilot trial evaluating an 123I-labeled 80-kilodalton engineered anticarcinoembryonic antigen antibody fragment (cT84.66 minibody) in patients with colorectal cancer. Clin. Canc. Res. 10(15):5014-21

Olafsen,T., Cheung, C.-C., Yazaki, P.J., Li, L., Sundaresan, G., Gambhir, S.S., Sherman, M.A., Williams, L.E., Shively, J.E., Raubitschek, A.A., and Wu, A.M. (2004) Covalent disulfide-linked anti-CEA diabody allows site-specific conjugation and radiolabeling for tumor targeting applications. Prot. Eng. Design & Selection 17:: 21-27.

   
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- David Geffen School of Medicine at UCLA -
- Department of Molecular and Medical Pharmacology -